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phycoerythrin-conjugated mouse anti-chicken cd8  (SouthernBiotech)


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    Structured Review

    SouthernBiotech phycoerythrin-conjugated mouse anti-chicken cd8
    Phycoerythrin Conjugated Mouse Anti Chicken Cd8, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phycoerythrin+conjugated+mouse+anti+chicken+cd8/Mouse+Anti-Chicken+Lambda-PE/pm39852969-353-58-63
    Average 93 stars, based on 10 article reviews
    phycoerythrin-conjugated mouse anti-chicken cd8 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Effects of Induced Moisture Loss in Chicken Embryos at Embryonic Day 18 and Post-hatch Immune Response During Salmonella enteritidis Lipopolysaccharide Challenge in Broilers
    Article Snippet: .. Single-cell suspensions from the spleen and thymus pools were enriched for lymphocytes by density centrifugation over Histopaque (1.077 g/ml, Sigma-Aldrich, St. Louis, MO, United States) for 15 min at 400 g . The cells were incubated with a 1:250 dilution of fluorescent-isothiocyanate conjugated mouse anti-chicken CD4 + (Southern Biotech, Birmingham, AL, United States); 1:450 dilution of phycoerythrin-conjugated mouse anti-chicken CD8 + (Southern Biotech, Birmingham, AL, United States), and 1:200 dilution of unlabeled mouse IgG for 15 min. .. The unbound antibodies were removed by centrifugation, and the percentages of CD4 + and CD8 + cells were analyzed using a flow cytometer (Guava EasyCyte, Millipore, Taunton, MA, United sates).

    Article Title: Subclinical Doses of Combined Fumonisins and Deoxynivalenol Predispose Clostridium perfringens– Inoculated Broilers to Necrotic Enteritis
    Article Snippet: .. In brief, single-cell suspensions from the spleen and cecal tonsils were enriched for mononuclear cells by density centrifugation over Histopaque (1.077 g/ml, Sigma-Aldrich, St. Louis, MO) for 15 min at 400 g. The cells were incubated with a 1:250 dilution of fluorescent-isothiocyanate conjugated mouse anti-chicken CD4 + (Southern Biotech, Birmingham, AL), 1:450 dilution of phycoerythrin-conjugated mouse anti-chicken CD8 + (Southern Biotech, Birmingham, AL), and 1:200 dilution of unlabeled mouse IgG for 15 min. .. The unbound antibodies were removed by centrifugation, the percentages of CD4 + and CD8 + cells were analyzed using a flow cytometer (Guava EasyCyte, Millipore, MA), and CD8 + : CD4 + ratio was calculated.

    Incubation:

    Article Title: Effects of Induced Moisture Loss in Chicken Embryos at Embryonic Day 18 and Post-hatch Immune Response During Salmonella enteritidis Lipopolysaccharide Challenge in Broilers
    Article Snippet: .. Single-cell suspensions from the spleen and thymus pools were enriched for lymphocytes by density centrifugation over Histopaque (1.077 g/ml, Sigma-Aldrich, St. Louis, MO, United States) for 15 min at 400 g . The cells were incubated with a 1:250 dilution of fluorescent-isothiocyanate conjugated mouse anti-chicken CD4 + (Southern Biotech, Birmingham, AL, United States); 1:450 dilution of phycoerythrin-conjugated mouse anti-chicken CD8 + (Southern Biotech, Birmingham, AL, United States), and 1:200 dilution of unlabeled mouse IgG for 15 min. .. The unbound antibodies were removed by centrifugation, and the percentages of CD4 + and CD8 + cells were analyzed using a flow cytometer (Guava EasyCyte, Millipore, Taunton, MA, United sates).

    Article Title: Subclinical Doses of Combined Fumonisins and Deoxynivalenol Predispose Clostridium perfringens– Inoculated Broilers to Necrotic Enteritis
    Article Snippet: .. In brief, single-cell suspensions from the spleen and cecal tonsils were enriched for mononuclear cells by density centrifugation over Histopaque (1.077 g/ml, Sigma-Aldrich, St. Louis, MO) for 15 min at 400 g. The cells were incubated with a 1:250 dilution of fluorescent-isothiocyanate conjugated mouse anti-chicken CD4 + (Southern Biotech, Birmingham, AL), 1:450 dilution of phycoerythrin-conjugated mouse anti-chicken CD8 + (Southern Biotech, Birmingham, AL), and 1:200 dilution of unlabeled mouse IgG for 15 min. .. The unbound antibodies were removed by centrifugation, the percentages of CD4 + and CD8 + cells were analyzed using a flow cytometer (Guava EasyCyte, Millipore, MA), and CD8 + : CD4 + ratio was calculated.

    Single Cell:

    Article Title: Subclinical Doses of Combined Fumonisins and Deoxynivalenol Predispose Clostridium perfringens– Inoculated Broilers to Necrotic Enteritis
    Article Snippet: .. In brief, single-cell suspensions from the spleen and cecal tonsils were enriched for mononuclear cells by density centrifugation over Histopaque (1.077 g/ml, Sigma-Aldrich, St. Louis, MO) for 15 min at 400 g. The cells were incubated with a 1:250 dilution of fluorescent-isothiocyanate conjugated mouse anti-chicken CD4 + (Southern Biotech, Birmingham, AL), 1:450 dilution of phycoerythrin-conjugated mouse anti-chicken CD8 + (Southern Biotech, Birmingham, AL), and 1:200 dilution of unlabeled mouse IgG for 15 min. .. The unbound antibodies were removed by centrifugation, the percentages of CD4 + and CD8 + cells were analyzed using a flow cytometer (Guava EasyCyte, Millipore, MA), and CD8 + : CD4 + ratio was calculated.



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    Effect of subclinical dose of FB + DON and E. maxima/C. perfringens challenge on the spleen and cecal tonsil <t>CD8</t> + : CD4 + ratio. Day-old chicks were distributed into four treatment groups: control, necrotic enteritis (NE), fumonisin + deoxynivalenol (FB + DON), and FB + DON + NE groups. Birds in the NE and FB + DON + NE groups received 2.5 × 10 3 Eimeria maxima oocyst per bird on day 14. All birds received 1 × 10 8 CFU/bird of Clostridium perfringens on days 19, 20, and 21. CD4 + and CD8 + cells were identified using fluorescent-linked anti-chicken CD4 and CD8 in a flow cytometer. Bars (+SEM) without a common superscript differ significantly ( p < 0.05). n = 8 (8 pens of 15 birds/pen).
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    Effect of subclinical dose of FB + DON and E. maxima/C. perfringens challenge on the spleen and cecal tonsil <t>CD8</t> + : CD4 + ratio. Day-old chicks were distributed into four treatment groups: control, necrotic enteritis (NE), fumonisin + deoxynivalenol (FB + DON), and FB + DON + NE groups. Birds in the NE and FB + DON + NE groups received 2.5 × 10 3 Eimeria maxima oocyst per bird on day 14. All birds received 1 × 10 8 CFU/bird of Clostridium perfringens on days 19, 20, and 21. CD4 + and CD8 + cells were identified using fluorescent-linked anti-chicken CD4 and CD8 in a flow cytometer. Bars (+SEM) without a common superscript differ significantly ( p < 0.05). n = 8 (8 pens of 15 birds/pen).
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    Image Search Results


    Effect of subclinical dose of FB + DON and E. maxima/C. perfringens challenge on the spleen and cecal tonsil CD8 + : CD4 + ratio. Day-old chicks were distributed into four treatment groups: control, necrotic enteritis (NE), fumonisin + deoxynivalenol (FB + DON), and FB + DON + NE groups. Birds in the NE and FB + DON + NE groups received 2.5 × 10 3 Eimeria maxima oocyst per bird on day 14. All birds received 1 × 10 8 CFU/bird of Clostridium perfringens on days 19, 20, and 21. CD4 + and CD8 + cells were identified using fluorescent-linked anti-chicken CD4 and CD8 in a flow cytometer. Bars (+SEM) without a common superscript differ significantly ( p < 0.05). n = 8 (8 pens of 15 birds/pen).

    Journal: Frontiers in Physiology

    Article Title: Subclinical Doses of Combined Fumonisins and Deoxynivalenol Predispose Clostridium perfringens– Inoculated Broilers to Necrotic Enteritis

    doi: 10.3389/fphys.2022.934660

    Figure Lengend Snippet: Effect of subclinical dose of FB + DON and E. maxima/C. perfringens challenge on the spleen and cecal tonsil CD8 + : CD4 + ratio. Day-old chicks were distributed into four treatment groups: control, necrotic enteritis (NE), fumonisin + deoxynivalenol (FB + DON), and FB + DON + NE groups. Birds in the NE and FB + DON + NE groups received 2.5 × 10 3 Eimeria maxima oocyst per bird on day 14. All birds received 1 × 10 8 CFU/bird of Clostridium perfringens on days 19, 20, and 21. CD4 + and CD8 + cells were identified using fluorescent-linked anti-chicken CD4 and CD8 in a flow cytometer. Bars (+SEM) without a common superscript differ significantly ( p < 0.05). n = 8 (8 pens of 15 birds/pen).

    Article Snippet: In brief, single-cell suspensions from the spleen and cecal tonsils were enriched for mononuclear cells by density centrifugation over Histopaque (1.077 g/ml, Sigma-Aldrich, St. Louis, MO) for 15 min at 400 g. The cells were incubated with a 1:250 dilution of fluorescent-isothiocyanate conjugated mouse anti-chicken CD4 + (Southern Biotech, Birmingham, AL), 1:450 dilution of phycoerythrin-conjugated mouse anti-chicken CD8 + (Southern Biotech, Birmingham, AL), and 1:200 dilution of unlabeled mouse IgG for 15 min.

    Techniques: Control, Flow Cytometry